David E. Roll, Ph.D
有机化学、生物化学和人类生物学名誉教授Education
Urbana, Illinois Ph.D. (1976) Biochemistry
University of Illinois M.S. (1972) Biochemistry
Harding College B.S. (1970) Chemistry (Searcy, Arkansas)
Biography
About Dr. Roll
I was born in Baltimore Maryland. 我父母决定搬到中西部去，所以我决定和他们一起搬. 我在密苏里州的堪萨斯城长大，然后进入阿肯色州的哈丁大学学习，在那里我获得了B.S. degree in chemistry. 我在伊利诺伊大学厄巴纳分校完成了生物化学硕士和博士学位. 我研究了感染劳斯肉瘤病毒的鸡成纤维细胞的质膜和细胞内的复合碳水化合物的结构. Graduate studies provided another bonus. 在实验室工作的时候，我遇到了我未来的妻子佩吉，美高梅mgm平台之间产生了默契. 美高梅mgm平台有五个孩子和两个孙子，婚姻仍然很幸福. 美高梅mgm平台搬到了匹兹堡大学医学院，在那里我研究了α -1抗胰蛋白酶缺乏症. 完成博士后工作后，我开始在罗伯茨卫斯理学院的化学系任教. 我对拓扑异构酶的合成和调控很感兴趣, particularly in Chlamydia bacteria, 从人血清和小牛血清中高效纯化α -1-抗胰蛋白酶. 此外，我正在从事涉及DNA身份测试的法医学研究项目. 我还在贝勒医学院完成了休假研究项目, the University of Rochester School of Medicine, 以及马里兰大学医学院荧光光谱研究中心.
Professional Affiliations
Alpha Chi Sigma Chemical Fraternity
American Scientific Affiliation
American Society for Biochemistry and Molecular Biology
National Association of Advisors for the Health Professions
Federation of American Scientists
Council of Undergraduate Research
Research Interests
Eukaryotic cell differentiation, animal cell membrane and cell surface carbohydrates, glycoprotein synthesis and secretion, viral cell transformation, topoisomerases and cell transformation, topoisomerase genes in Chlamydia.
Grants Funded
"Recombinant DNA Technology in the Undergraduate Curriculum," National Science Foundation, College Science Instrumentation Program, Matching Grant $6,000, NSF; $6,000, RWC (1985).
"Cell Biology Laboratory for Advanced Undergraduates," National Science Foundation, College Science Instrumentation Program, Matching Grant $11,000, NSF; $11,000, RWC, (1991).
衣原体中可能的新化疗靶点的鉴定," Academic Research Enhancement Award, National Institutes of Health, $93,610, (1996).
“Introducing FT-NMR in the Undergraduate Curriculum,” National Science Foundation, CCLI Adaptation and Implementation program, Matching Grant $27,187.50, (2000).
Patent
共同发明者:David Roll, Ignacy Grczynski, Joanna Malicka, zygmont Gryczynski和Joseph R. Lakowicz. 专利公开JRL-63“金属胶体波长比率散射传感器”
Publications and Abstracts
Weber, M.J. Hale, R.H., and Roll, D.E., 蛋白酶活性在劳斯肉瘤病毒恶性转化中的作用,"Proteases and Biological Control, pp. 915-930, Cold Spring Harbor Laboratory, 1975.
Roll, D.E. and Conrad, H.E., 《培养动物细胞中主要碳水化合物群的定量放射色谱分析," Anal. Biochem., 77, 397-412 (1977).
Roll, D.E., Aguanno, J., Coffee, C.J. and Glew, R.H., 正常α -1-抗胰蛋白酶和s -变异α -1-抗胰蛋白酶的碳水化合物和氨基酸组成比较," Biochem. Biophys. Acta, 532, 171-178 (1978).
Leonard, J., Hale, A., Roll, D., Conrad, H.E. and Weber, M., 正常和劳斯肉瘤病毒转化细胞中细胞碳水化合物的周转," Cancer Research, 38, 185-188 (1978).
Roll, D.E., Aguanno, J. and Glew, R.H., n -乙酰神经氨酸与α -1-抗胰蛋白酶糖肽的附着研究," Fed. Proc. 37, Abstract #1828 (1978).
Roll, D.E., Aguanno, J., Coffee, C.J., and Glew, R.H., α -1-抗胰蛋白酶的溴化氰衍生糖肽片段的分离和表征," J. Biol. Chem., 253, 6992-6996 (1978).
Aguanno, J., Roll, D.E., Coffee, C.J., and Glew, R.H., n -乙酰神经氨酸与α -1-抗胰蛋白酶糖肽的附着研究," J. Biol. Chem., 253, 6997-7004 (1978).
Roll, D.E., Weber, M.J., and Conrad, H.E., 生长条件对鸡胚成纤维细胞主要类碳水化合物含量的影响," Cancer Research, 39, 2550-2555 (1979).
Durban, E., Roll, D., Beckner, G. and Busch, H.核dna结合磷酸化蛋白110/8的纯化与鉴定.4 Found in Tumor and Fetal Tissues," Fed. Proc. 39, Abstract #1856 (1980).
Durban, E., Roll, D., Beckner, G., and Busch, H., 胎儿和肿瘤组织中核dna结合磷酸化蛋白的纯化和特性," Cancer research, 41, 537-545 (1981).
Roll, D.E., and Glew, R.H.，“大鼠α -1-抗胰蛋白酶的分离与鉴定”，J. Biol. Chem. 256, 8190-8196 (1981).
Durban, E., Mills, S., Roll, D. and Busch, H.纯化肝癌拓扑异构酶I的磷酸化，《生物化学》. Biophys, Res. Comm., 111, 897-905 (1983).
Rao, M.R.S., Ahr, V.S., Spohn, W.H., Durban, E., Roll, D. and Busch, H.，“一种新的酸性(p14) 19千道尔顿核仁磷酸化蛋白，”J. 细胞生物学，1997，美国细胞生物学学会文摘# 527,140a (1983).
McRorie, D.K., Rao, M.R.S., Goldknopf, I.L., Harty, T.P., Roll, D.E., Ahn, V.S., and Busch, H., "Purification and Partial Characterization of a 19KD/pI 4.5 Nucleolar Phosphoprotein," Biochem. Biophys, Res. Comm., 122, 47-55 (1984).
Roll, D. and Durban, E., 拓扑异构酶I酪氨酸磷酸化位点和dna相互作用位点," American Society of Biological Chemists. Abstract #1707, (1986).
Roll, D., Software Review of "Simulation of Hemoglobin Function," J. Chem. Ed., 65, A113-114 (1988).
Roll, D., "Recombinant DNA Technology in the Undergraduate Curriculum,的摘要和海报提交给由美国科学协会主办的基因剪接会议, July, 1988.
Bramucci, M., Roll, D., Cook, R. and Durban, E., 重组DNA文库表达一致表位结构的鉴定," Molecular Immunology, 26, 741-748 (1989).
Roll, D., Murphy, B.J., Laderoute, K.R., Sutherland, R.M. and Smith, H.C., 氧调节80KDa蛋白和葡萄糖调节78KDa蛋白是相同的," Molecular and Cellular Biochemistry, 103, 141-148 (1991).
Daschev, C., Roll, D.E., Bavoil, P.M., 衣原体主要外膜蛋白在大肠杆菌中的表达和易位," Microbial Pathogenesis, 15, (1993).
Roll, D., Hsia, R., Bavoil, P.M.，《美高梅mgm平台》.《美高梅mgm平台》，Orfilia等. al., (eds.), Societo' Editrice Esculapio, Bologna, 263-266 (1994).
Adams, R., Roll, D.(2)《美高梅mgm平台》.在第九届人类衣原体感染国际研讨会上发表的摘要和海报, June 21-26, 1998, Napa Valley, California.
Geddes, C. D., Parfenov, A. Gryczynski, I., Malicka, J., Roll, D.,and Lakowicz, J. R., 金属增强荧光的分形银结构:超亮表面分析和基于芯片实验室的纳米技术的应用,” Journal of Fluorescence, 13(2), 123-128, 2003.
Roll, D., Malicka, J., Gryczynski, I., Gryczynski, Z., and Lakowicz, J. R., “Metallic Colloid Wavelength-Ratiometric Scattering Sensors,” Analytical Chemistry 75(14), 3440-3445 (2003).
Lakowicz, J.R., Malicka, J., Gryczynski, Z., Roll, D., Huang, J., Geddes, C. D., and Gryczynski, I. , 利用金属纳米颗粒提高荧光检测的灵敏度,” PharmaGenomics, 3(3), 38-46 (2003).
Geddes, C.D., Parfenov, A., Roll, D., Fang, J., and Lakowicz, J.R., 金属增强荧光用银的电化学和激光沉积,” Langmuir 19(15), 6236-6241 (2003).
Geddes, C.D., Parfenov, A., Roll, D. Gryczynski, I., Malicka, J., Lakowicz, J. R., 金属增强荧光的银分形结构:增强荧光强度和增加探针光稳定性,” Journal of Fluorescence 13(3), 267-276 (2003).
Geddes, C. D., Roll, D., Parfenov, A., and Lakowicz, J. R., “Noble-Metal Surfaces for use in Metal-Enhanced Fluorescence,” Poster #B705, 发表于第47届生物物理学会年会上, San Antonio, Texas, March 1-5, 2003.
Geddes, C. D., Parfenov, A., Roldd, D., and Lakowicz, J. R., 粗化铂电极上吲哚菁绿的增强光稳定性:金属增强荧光,” Journal of Fluorescence 13(6), 453-457 (2003).
Geddes C. D., Parfenov, A., Roll, D., and Lakowicz, J. R.，“用于金属增强荧光的粗糙银电极”，光谱化学学报A., 60 (8-9), 1977-1983, (2004).
Zhang, J., Roll, D., Geddes, C. D., and Lakowicz, J. R., 银纳米颗粒-葡聚糖加合物与魔豆蛋白A的聚集和与葡萄糖的竞争置换,” Journal of Physical Chemistry B, 108 (32), 12210-12214 (2004).